abstract：:Amylose spherocrystals of A-type were grown by mixing ethanol with hot aqueous solutions of short chain amylose followed by slow cooling to 4 degrees C. The spherocrystals which had a diameter of the order of 10 microns were observed by scanning electron microscopy and analysed by X-ray diffraction. They were also cro...

journal_title：International journal of biological macromolecules

authors： Helbert W,Chanzy H,Planchot V,Buléon A,Colonna P

更新日期：1993-06-01 00:00:00

abstract：:Miniature crystal models of cellulose and other carbohydrates were evaluated with the molecular mechanics program MM3. The models consisted of groups of 24 to 32 monosaccharide residues, with the models of mono- and disaccharides based on well-established, single-crystal work. Structures of the cellulose forms and cel...

journal_title：International journal of biological macromolecules

authors： French AD,Miller DP,Aabloo A

更新日期：1993-02-01 00:00:00

abstract：:Detailed characterization of enzyme susceptibility of bacterial cellulose containing N-acetylglucosamine (GlcNAc) residues (N-AcGBC) which possess high susceptibility for cellulase and lysozyme and slight susceptibility for chitinase was studied. Turbidimetric lysozyme assay of N-AcGBC showed that (i) the susceptibili...

journal_title：International journal of biological macromolecules

authors： Ogawa R,Miura Y,Tokura S,Koriyama T

更新日期：1992-12-01 00:00:00

abstract：:The interaction of two anthraquinone dyes, bromaminic acid 1-amino-4-bromo anthraquinone-2-sulphonic acid (BA) and 1-amino-4-(4'-aminophenylamino)-anthraquinone-2,3'-disulphonic acid (ASSO), with poly-L-lysine (PLL), poly-L-ornithine (PLO) and poly-L-arginine (PLA) has been studied. The flexibility of the side chain (...

journal_title：International journal of biological macromolecules

authors： Ratna MA,Atreyi M,Rao MV

更新日期：1992-08-01 00:00:00

abstract：:In many skeletal support systems of plants and animals, cellulose, chitin, and collagen occur in the form of microfibrils ordered in a chiral nematic fashion (helicoids). However, these structures remain poorly understood due to the many constituents present in biological tissues. Here we report an in vitro system tha...

journal_title：International journal of biological macromolecules

authors： Revol JF,Bradford H,Giasson J,Marchessault RH,Gray DG

更新日期：1992-06-01 00:00:00

abstract：:Limited proteolysis of streptokinase (Sk) by trypsin and thermolysin was performed under various incubation conditions and analysed by polyacrylamide gel electrophoresis. Several fragments (Sk1, Tr27, Tr17, Th26, and Th16) were isolated and characterized further. The N-terminal sequences of Tr27, Tr17, Th26, Th16 and ...

journal_title：International journal of biological macromolecules

authors： Misselwitz R,Kraft R,Kostka S,Fabian H,Welfle K,Pfeil W,Welfle H,Gerlach D

更新日期：1992-04-01 00:00:00

abstract：:The formation of poly(beta-hydroxybutyrate), PHB, by Rhodobacter sphaeroides and Alcaligenes eutrophus was studied using the following carbon sources and solvents: (1), acetate in H2O; (2), D3-acetate in H2O; (3), acetate in 90 to 92% D2O; and (4), D3-acetate in 90 to 92% D2O. The growth of Rb. sphaeroides cultured un...

journal_title：International journal of biological macromolecules

authors： Gross RA,Ulmer HW,Lenz RW,Tshudy DJ,Uden PC,Brandt H,Fuller RC

更新日期：1992-02-01 00:00:00

abstract：:This review summarizes some of the results obtained from the crystal structure determination of viruses. The structural similarities of viral coat proteins is discussed, as well as some structural results related to the regulation of viral disassembly. ...

journal_title：International journal of biological macromolecules

authors： Liljas L

更新日期：1991-10-01 00:00:00

abstract：:Hydrodynamic data, i.e. intrinsic viscosity and sedimentation coefficient, for denatured globular proteins in 6 M guanidinium chloride have been re-analysed in terms of the Yamakawa-Fujii theory of the wormlike cylinder model. Molecular parameters thus obtained ((mean value of R2 0/M) infinity, the Kuhn statistical se...

journal_title：International journal of biological macromolecules

authors： Bohdanecký M,Petrus V

更新日期：1991-08-01 00:00:00

abstract：:The principal advantage of the n.m.r. imaging method lies in the specific contrasts which are available. In this work we describe the use of velocity and diffusion contrast methods in biophysical applications and at microscopic spatial resolution. In the first example, involving water-protein interactions, the relatio...

journal_title：International journal of biological macromolecules

authors： Back PJ,Coy A,Xia Y,Callaghan PT,Diamante LM,Umbach SL

更新日期：1991-06-01 00:00:00

abstract：:This paper concerns the characterization of the chemical structure of a DP3 glucosamine oligomer. The assignments of nearly all protons are reported. Variations of 1H chemical shifts with pD and temperature are correlated to the pKa of amino groups, and not with substantial conformational changes. ...

journal_title：International journal of biological macromolecules

authors： Domard A,Gey C,Taravel F

更新日期：1991-04-01 00:00:00

abstract：:Using samples previously degraded by autohydrolysis, it is possible to obtain valuable low-field 13C-n.m.r. spectra of samples of kappa/iota carrageenans at room temperature. Spectroscopy of samples extracted at different stages of autohydrolysis helps to determine the mechanism of the reaction and the presence of low...

journal_title：International journal of biological macromolecules

authors： Stortz CA,Cerezo AS

更新日期：1991-04-01 00:00:00

abstract：:Time-dependent intermolecular sulphydryl-disulphide interchange involving beta-lactoglobulin adsorbed at the oil-water interface in n-tetradecane-in-water emulsions (10 wt% oil, 0.5 wt% protein, pH 7.0) has been investigated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). While only monome...

journal_title：International journal of biological macromolecules

authors： Dickinson E,Matsumura Y

更新日期：1991-02-01 00:00:00

abstract：:A systematic modelling for the branching point of amylopectin, on the basis of the double-helical structure proposed for amylose polymorphs was performed. A 'several steps' approach was used with increasing sophistication in the refinement, combining the use of geometrical criteria and molecular mechanics calculations...

journal_title：International journal of biological macromolecules

authors： Buléon A,Tran V

更新日期：1990-12-01 00:00:00

abstract：:The binding of acetazolamide to human carbonic anhydrase II (HCA II) has been investigated by X-ray crystallography. The atomic positions of the enzyme inhibitor complex have been refined at 1.9 A resolution using the least squares refinement program package PROLSQ. The crystallographic R-factor is 17.6%. The bound in...

journal_title：International journal of biological macromolecules

authors： Vidgren J,Liljas A,Walker NP

更新日期：1990-12-01 00:00:00

abstract：:A new model of secondary and tertiary structure of higher plant 5S RNA is proposed. It consists of three helical domains: domain alpha includes stem I; domain beta contains stems II and III and loops B and C; domain gamma consists of stems IV and V and loops D and E. Except for, presumably, a canonical RNA-A like doma...

journal_title：International journal of biological macromolecules

authors： Joachimiak A,Nalaskowska M,Barciszewska M,Barciszewski J,Mashkova T

更新日期：1990-10-01 00:00:00

abstract：:Poly-L-lysine (PLL) and bovine serum albumin were used as models in the investigation of non-enzymatic glycation and browning reaction by circular dichroism measurements. Different sugars (glucose, glucose-6-P and ribose) induced different extents of reaction and produced different chromophores that absorbed light in ...

journal_title：International journal of biological macromolecules

authors： Liang JN

更新日期：1990-08-01 00:00:00

abstract：:We report the chemical characterization and the relationship between the physicochemical properties and conformational change of a succinoglycan polysaccharide produced by Pseudomonas sp, NCIB 11592. The expected chemical structure is confirmed, with a ratio of D-glucose: D-galactose: pyruvate: succinate of 7:1:1:1. T...

journal_title：International journal of biological macromolecules

authors： Gravanis G,Milas M,Rinaudo M,Clarke-Sturman AJ

更新日期：1990-06-01 00:00:00

abstract：:The production of two extracellular capsular heteropolysaccharides by two different Acinetobacter strains has been studied in separate controlled fermentation processes with a view to their industrial applications as specific dispersing agents. The first, emulsan, is an extracellular polyanionic amphipathic heteropoly...

journal_title：International journal of biological macromolecules

authors： Shabtai Y

更新日期：1990-04-01 00:00:00

abstract：:Photolysis of HbNO has been studied from 6.2 K to 15.5 K by electron spin resonance during and after continuous illumination. Non-exponential kinetics of both dissociation and reassociation of NO was observed. The prolonged illumination separates the fast and slow ligands. This picture is consistent with NO tunnelling...

journal_title：International journal of biological macromolecules

authors： Linhares MP,el-Jaick LJ,Bemski G,Wajnberg E

更新日期：1990-02-01 00:00:00

abstract：:Sequential glycopeptides [Thr(beta-D-galactose)-Ala-Ala]n, with n ranging from 2 to 7, as models of natural antifreeze glycoproteins were synthesized by the continuous flow, solid phase procedure. The conformational properties of these materials in solution were investigated by c.d. and 1H-n.m.r. spectroscopy. In aque...

journal_title：International journal of biological macromolecules

authors： Filira F,Biondi L,Scolaro B,Foffani MT,Mammi S,Peggion E,Rocchi R

更新日期：1990-02-01 00:00:00

abstract：:A tetrapetide containing an Aib residue, Boc-Asn-Aib-Thr-Aib-OMe, was synthesized as a peptide model for the N-glycosylation site in N-glycoproteins. Backbone conformation of the peptide and possible intramolecular interaction between the Asn and Thr side chains were elucidated by means of n.m.r. spectroscopy. Tempera...

journal_title：International journal of biological macromolecules

authors： Ishii H,Nadaoka O,Mimura Y,Inoue Y,Chûjô R

更新日期：1989-12-01 00:00:00

abstract：:The Raman spectra of kappa and iota carrageenan, a desulphated furcellaran and a series of oligomers have been compared in the region 700-1500 cm-1. Spectral differences depending on the amount and the location of the sulphate group on the ring, the chain length, the nature of the counterion and the conformation are d...

journal_title：International journal of biological macromolecules

authors： Malfait T,Van Dael H,Van Cauwelaert F

更新日期：1989-10-01 00:00:00

abstract：:Citrus pectins with degrees of methylation between 30 and 72% were carefully characterized in order to determine their charge density and molecular weight distribution, the content in galacturonic acid and in neutral sugars, the degree of methylation and acetylation. Using enzymic degradation it has been found that pe...

journal_title：International journal of biological macromolecules

authors： Axelos MA,Thibault JF,Lefebvre J

更新日期：1989-06-01 00:00:00

abstract：:The amino acid sequences of chick and slime mould alpha-actinin each contain four repeats of approximately 122 residues. These repeats are homologous to the 18-22 repeats, each of approximately 106 residues, found in the alpha and beta subunits of spectrin and fodrin, and to the multiple repeats of approximately 110 r...

journal_title：International journal of biological macromolecules

authors： Davison MD,Baron MD,Critchley DR,Wootton JC

更新日期：1989-04-01 00:00:00

abstract：:Beta-lactoglobulin has been found to be a member of a super family of protein that bind specific ligands and which share common features in their amino acid sequences. Here we show that these features are grouped spatially on the surface of the proteins and suggest that they may be concerned with binding to cell-surfa...

journal_title：International journal of biological macromolecules

authors： North AC

更新日期：1989-02-01 00:00:00